C# Will reflection create multiple instance of a singleton in my assembly?

I am creating a c# based automated testing framework and have configuration files that link test case methods and class names to requirement IDs. The framework will create a TestPlan that is a list of multiple test cases that will executed one after another.

Since there are thousands of testcases (and more all the time) I want to call these classes and methods by reflection, but I am concerned that these methods will create singleton instances of supporting classes that will hang around after the method returns that won't be reused when the next test method is called by reflection.

My question is: will each call use the same static instance in the assembly? Or will each call create a new instance of the singleton? Without reflection in the mix it is a simple answer, but with it ... I am not certain.